2-amino-5-nitrothiazole physical and chemical properties The operation process is many, novice operation will inevitably make mistakes. Many of these faults are caused by poor details, and there are many ways to reduce them, such as speaking less during experiments and avoiding saliva falling into the enzyme marker. Of course, you also have to learn to explore the problem and find out the cause. So how do we fix the error of the 2-amino-5-nitrothiazole physical and chemical properties test?

① : Evaluating the practicality of the reagent and whether the reagent is stable or not is important for the concave and convex head of the rate. Before the reagent is used, Yin and Yang control and sample repeated comparison test should be carried out to determine that the reagent meets the requirements before it can be used.

② : Read the instructions carefully before operation, and strictly follow the requirements of the instructions for standardized operation. Different batches of reagents should not be mixed. Where it is worth improving, it can be improved only after repeated testing to confirm the establishment, such as the grasp of the number of washing boards.

③ : Adding samples should be fast. If the sample is not added, the amount of enzyme products can not be confirmed, which directly affects the color development results. In addition, the depth of color development and the determination of A value are related to the amount of color development agent and termination liquid added, so the sample should be carefully added. The test requiring the end of the sample addition within a certain time, if the sample addition is slow, there will be errors, and the reagent is exposed for a long time, especially when the room temperature is too high, the retention period will be shortened or even ineffective.

④ : Inspection technicians should have the basic quality and practical experience to engage in laboratory operation. Be able to skillfully operate test instruments and instruments, have the ability to summarize and analyze problems, and timely and properly solve unexpected situations in the test.

⑤ : Use the calibrated micro pipette to eliminate natural errors. Pipette or not is particularly important for quantitative detection.

6: Strictly grasp the color development time, the color development time is too short, after the termination of the reaction by adding the termination liquid, the amount of substrate binding is too small, and it is easy to show false negative. Color development beyond the time required for color development should be judged as false positive, which may be related to the reagent itself. Add color development agent immediately after the color, not positive, which may be the result of background color development.

7: Wash *, wash plate is not *, enzyme binding background color will show false positive. In addition, the washing liquid should be fresh, used now, and the stale washing liquid will show a background increase phenomenon, and may also show false positives.

⑧ : Strict control of reaction time, reaction time is too long, enzyme inactivation; The reaction time is too short, the enzyme conjugate cannot fully combine with the microbial antigen and antibody in the serum, the structure of the product is loose and not strong, and it is easy to wash off, which may form a false negative.

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