Fluorescein isothiocyanate (FITC) is a commonly used fluorescent dye in various biological applications. Its utility largely depends on its fluorescence properties, particularly the wavelength at which it emits light.
What is FITC?
FITC is a fluorescent dye that attaches to proteins, nucleic acids, and other molecules, allowing them to be visualized under a fluorescence microscope. It is widely used in research and clinical diagnostics due to its bright and stable fluorescence.
FITC Emission and Excitation Wavelengths
FITC exhibits specific wavelengths for excitation and emission:
- Excitation Wavelength: FITC is excited by light in the ultraviolet to blue range, typically around 495 nm. This means that when exposed to light of this wavelength, FITC absorbs the energy and becomes excited.
- Emission Wavelength: Once excited, FITC emits light at a longer wavelength, generally around 520 nm. This emission is visible as green fluorescence, making it easy to detect using appropriate filters in microscopy.
Applications of FITC Wavelength
The specific wavelengths of FITC are crucial for its applications. Researchers use FITC to label and track molecules within cells or tissues. The distinct emission peak at 520 nm allows for clear differentiation from other fluorescent markers.
Conclusion
Understanding the FITC wavelength is essential for effective use in scientific research. By utilizing its excitation and emission properties, researchers can achieve precise and reliable results in various applications, from cell biology to medical diagnostics.
